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Issue 17, 2011

Dissociation techniques in mass spectrometry-based proteomics

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Abstract

The field of proteomics, the large-scale analysis of proteins, has undergone a huge expansion over the past decade. Mass spectrometry-based proteomics relies on the dissociation of peptide and/or protein ions to provide information on primary sequence and sites of post-translational modifications. Fragmentation techniques include collision-induced dissociation, electron capture dissociation and electron transfer dissociation. Here, we describe each of these techniques and their use in proteomics. The principles, advantages, limitations, and applications are discussed.

Graphical abstract: Dissociation techniques in mass spectrometry-based proteomics

Article information


Submitted
16 Dec 2010
Accepted
15 May 2011
First published
23 Jun 2011

Analyst, 2011,136, 3419-3429
Article type
Minireview

Dissociation techniques in mass spectrometry-based proteomics

A. W. Jones and H. J. Cooper, Analyst, 2011, 136, 3419 DOI: 10.1039/C0AN01011A

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