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Issue 17, 2011
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Dissociation techniques in mass spectrometry-based proteomics

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Abstract

The field of proteomics, the large-scale analysis of proteins, has undergone a huge expansion over the past decade. Mass spectrometry-based proteomics relies on the dissociation of peptide and/or protein ions to provide information on primary sequence and sites of post-translational modifications. Fragmentation techniques include collision-induced dissociation, electron capture dissociation and electron transfer dissociation. Here, we describe each of these techniques and their use in proteomics. The principles, advantages, limitations, and applications are discussed.

Graphical abstract: Dissociation techniques in mass spectrometry-based proteomics

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Publication details

The article was received on 16 Dec 2010, accepted on 15 May 2011 and first published on 23 Jun 2011


Article type: Minireview
DOI: 10.1039/C0AN01011A
Citation: Analyst, 2011,136, 3419-3429
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    Dissociation techniques in mass spectrometry-based proteomics

    A. W. Jones and H. J. Cooper, Analyst, 2011, 136, 3419
    DOI: 10.1039/C0AN01011A

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