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Issue 9, 2011
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An effective real-time colorimeteric sensor for sensitive and selective detection of cysteine under physiological conditions

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Abstract

In this contribution, a new, real-time and sensitive colorimetric sensor, di-N-methyl-N-hydroxyethylaniline squaraine (SQ), has been identified and synthesized for cysteine analysis based on its ΔA in neutral aqueous medium (pH ≈ 7.5). The proposed method was applied to analyse synthetic amino acid samples and human serum samples. The results show that the linear range of cysteine detection in aqueous medium at pH ≈ 7.5 is 10∼700 nmol L−1 with a correlation coefficient (R) of 0.9984 and a limit of detection (3σ, n = 20) of 3.9 nmol L−1. The relative standard deviation (RSD) for cysteine detection was lower than 4.1% (n = 5). The proposed method possesses the advantages of simplicity, rapidity, high selectivity and sensitivity. This makes it possible, for the first time, the real-time detection of cysteine under normal physiological conditions.

Graphical abstract: An effective real-time colorimeteric sensor for sensitive and selective detection of cysteine under physiological conditions

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Article information


Submitted
25 Nov 2010
Accepted
22 Feb 2011
First published
11 Mar 2011

Analyst, 2011,136, 1916-1921
Article type
Paper

An effective real-time colorimeteric sensor for sensitive and selective detection of cysteine under physiological conditions

Z. Yan, S. Guang, H. Xu and X. Liu, Analyst, 2011, 136, 1916
DOI: 10.1039/C0AN00943A

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