Issue 4, 2011
From the journal:

Analyst

The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies†

Hengyao Hu,‡a   Hao Yang,‡a   Ding Li,b   Kan Wang,a   Jing Ruan,a   Xueqing Zhang,a   Jun Chen,a   Chenchen Bao,a   Jiajia Ji,a   Donglu Shiac  and  Daxiang Cui*a  

* Corresponding authors

a Department of Bio-nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800Dongchuan Road, Shanghai, P.R. China
E-mail: dxcui@sjtu.edu.cn

b Center of biological diagnosis and therapy, No. 261 Hospital of PLA, Beijing, P.R. China

c Department of Chemical and Materials Engineering, University of Cincinnati, Cincinnati, OH, USA

Abstract

Magnetic nanoclusters (MNCs) were synthesized in a one-pot process, carboxylic MNCs and dual-functional protein were prepared and used to capture hepatitis B virus surface antibodies (anti-HBs) in simulated diseased oral mucosal transudate (OMT) samples. The specific substrate of dual-functional protein, dual-labeled double-chained DNA molecules, based on Fluorescence Resonance Energy Transfer (FRET), was used to amplify the detection signal and the detection limit of 0.1 ng mL−1 of anti-HBs monoclonal antibodies was achieved. Combination MNCs with dual-functional protein enables the noninvasive detection of hepatitis B virus (HBV) surface antibodies in OMT samples, showing promise as a diagnostic tool for the OMT diagnosis of infectious diseases with sensitive, specific and facile capabilities.

Graphical abstract: The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C0AN00517G
Article type
Communication
Submitted
15 Jul 2010
Accepted
20 Oct 2010
First published
03 Nov 2010

Analyst, 2011,136, 679-683

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The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies

H. Hu, H. Yang, D. Li, K. Wang, J. Ruan, X. Zhang, J. Chen, C. Bao, J. Ji, D. Shi and D. Cui, Analyst, 2011, 136, 679 DOI: 10.1039/C0AN00517G

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