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Issue 6, 2011
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Hydrogen-bonded LbL shells for living cell surface engineering

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We report on the design of cytocompatible synthetic shells from highly permeable, hydrogen-bonded multilayers for cell surface engineering with preservation of long-term cell functioning. In contrast to traditional polyelectrolyte layer-by-layer (LbL) systems, shells suggested here are based on hydrogen bonding allowing gentle cell encapsulation using non-toxic, non-ionic and biocompatible components such as poly(N-vinylpyrrolidone) (PVPON) and tannic acid (TA) which were earlier exploited on abiotic surfaces but never assembled on cell surfaces. Here, we demonstrate that these LbL shells with higher diffusion facilitate outstanding cell survivability reaching 79% in contrast to only 20% viability level achieved with ionically paired coatings. We suggest that the drastic increase in cell viability and preservation of cell functioning after coating with synthetic shell stems from the minimal exposure of the cells to toxic polycations and high shell permeability.

Graphical abstract: Hydrogen-bonded LbL shells for living cell surface engineering

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Article information

28 Sep 2010
17 Nov 2010
First published
23 Dec 2010

Soft Matter, 2011,7, 2364-2372
Article type

Hydrogen-bonded LbL shells for living cell surface engineering

V. Kozlovskaya, S. Harbaugh, I. Drachuk, O. Shchepelina, N. Kelley-Loughnane, M. Stone and V. V. Tsukruk, Soft Matter, 2011, 7, 2364
DOI: 10.1039/C0SM01070G

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