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Issue 14, 2011
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Molecular mechanisms of phosphatidylcholine monolayer solidification due to hydroxyl radicals

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Abstract

The reaction of L-α-1,2-dipalmitoylphosphatidylcholine (DPPC) with hydroxyl radicals (HO˙ from Fenton solutions) was investigated using monolayer techniques: isotherms, infrared reflection absorption spectroscopy (IRRAS), grazing incidence X-ray diffraction, X-ray reflection and fluorescence microscopy. The DPPC monolayer is attacked with different HO˙ concentrations. The decrease in the lateral pressure was used as a measure of the efficiency of the HO˙ attack. With increasing HO˙ concentration, the plateau region in the isotherm was shifted to a lower surface pressure; eventually it disappeared. Fluorescence microscopy during the HO˙ attack showed that new domains in the condensed phase nucleate immediately. With isotherms and X-ray diffraction we found that the monolayer can be compressed to smaller molecular areas, with a reduced tilt angle of the alkyl chains. IRRAS experiments indicated a partial cleavage of the head group leading to a reduced head group size.

Graphical abstract: Molecular mechanisms of phosphatidylcholine monolayer solidification due to hydroxyl radicals

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Article information


Submitted
21 Feb 2011
Accepted
06 May 2011
First published
08 Jun 2011

Soft Matter, 2011,7, 6467-6476
Article type
Paper

Molecular mechanisms of phosphatidylcholine monolayer solidification due to hydroxyl radicals

A. Gröning, H. Ahrens, T. Ortmann, F. Lawrenz, G. Brezesinski, F. Scholz and C. A. Helm, Soft Matter, 2011, 7, 6467
DOI: 10.1039/C1SM05312D

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