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Issue 8, 2011
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Compartment size dependence of performance of polymerase chain reaction inside giant vesicles

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Abstract

The amplification of a 1229 bp template DNA that encoded the green fluorescent protein (GFP) was conducted in cell-sized giant vesicles (GVs, ϕ > 1 µm) using a real-time polymerase chain reaction (PCR) technique. The proportion of PCR-proceeded GV reached up to 16% of all GVs. The dependence of PCR on GV size was elucidated by flow cytometry analysis.

Graphical abstract: Compartment size dependence of performance of polymerase chain reaction inside giant vesicles

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Supplementary files

Article information


Submitted
10 Dec 2010
Accepted
15 Feb 2011
First published
11 Mar 2011

Soft Matter, 2011,7, 3750-3753
Article type
Communication

Compartment size dependence of performance of polymerase chain reaction inside giant vesicles

K. Shohda, M. Tamura, Y. Kageyama, K. Suzuki, A. Suyama and T. Sugawara, Soft Matter, 2011, 7, 3750
DOI: 10.1039/C0SM01463J

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