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Issue 3, 2011
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Genetically encoding an aliphatic diazirine for protein photocrosslinking

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Abstract

Photocrosslinking is an important approach that allows discovery and detailed investigation of proteinprotein, proteinoligonucleotide, and protein–small molecule interactions with high temporal and spatial resolution. A major limitation to the universal application of this methodology is the site-specific introduction of efficient aliphatic photocrosslinking probes into proteins of interest. Here, we report a novel aliphatic diazirine amino acid and its genetically encoded, site-specific incorporation into proteins in bacterial and mammalian cells. Furthermore, we demonstrate efficient photocrosslinking of a test proteinin vitro and in vivo.

Graphical abstract: Genetically encoding an aliphatic diazirine for protein photocrosslinking

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Article information


Submitted
15 Jul 2010
Accepted
15 Nov 2010
First published
01 Dec 2010

Chem. Sci., 2011,2, 480-483
Article type
Edge Article

Genetically encoding an aliphatic diazirine for protein photocrosslinking

C. Chou, R. Uprety, L. Davis, J. W. Chin and A. Deiters, Chem. Sci., 2011, 2, 480
DOI: 10.1039/C0SC00373E

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