Jump to main content
Jump to site search

Issue 7, 2011
Previous Article Next Article

DNA hybridization of pathogenicity island of vancomycin-resistant Enterococcus faecalis with discretely functionalized gold nanoparticles in organic solvent mixtures

Author affiliations

Abstract

Short single-strand (ss) DNA sensors that involve mono-functionalized gold nanoparticles are reported for the first time. By way of an example, the hybridization and detection of a 10 base-pair oligonucleotide (TGACTCTTAA), which relates to the pathogenicity island of vancomycin-resistant Enterococcus faecalis, has been demonstrated in the organic solvent mixture (DMF/CHCl3/H2O/NaCl) at sub-nanomole concentrations. The formation of the resulting double-strand (ds) DNA–AuNP complexes which involve either tail-to-tail or head-to-head alignment, are sensitive to salt (NaCl) and solvent (CHCl3) concentrations. By UV/visible absorption measurements, it has been found that the melting temperature of the dsDNA–AuNP complexes increases with increasing salt concentrations as well as the chloroform ratio, for both tail-to-tail and head-to-head alignments. Transmission electron microscopic images of the dsDNA–AuNP complexes with mono-functionalized AuNPs reveal significant amounts of dimeric AuNP species while that of randomly functionalized AuNPs reveal an agglomerated AuNP nanostructures.

Graphical abstract: DNA hybridization of pathogenicity island of vancomycin-resistant Enterococcus faecalis with discretely functionalized gold nanoparticles in organic solvent mixtures

Back to tab navigation

Article information


Submitted
14 Jun 2011
Accepted
14 Aug 2011
First published
06 Oct 2011

RSC Adv., 2011,1, 1342-1348
Article type
Paper

DNA hybridization of pathogenicity island of vancomycin-resistant Enterococcus faecalis with discretely functionalized gold nanoparticles in organic solvent mixtures

C. Chak, J. M. Y. Lai, K. W. Y. Sham, C. H. K. Cheng and K. C. Leung, RSC Adv., 2011, 1, 1342
DOI: 10.1039/C1RA00304F

Social activity

Search articles by author

Spotlight

Advertisements