Issue 4, 2011
From the journal:

RSC Advances

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells†

Karin Y. Chumbimuni-Torres,a   Ramon E. Coronado,b   Adelphe M. Mfuh,a   Carlos Castro-Guerrero,c   Maria Fernanda Silva,d   George R. Negrete,a   Rena Biziosb  and  Carlos D. Garcia*a  

* Corresponding authors

a Department of Chemistry, The University of Texas at San Antonio, TX, USA
E-mail: carlos.garcia@utsa.edu
Tel: (210) 458-5774

b Department of Biomedical Engineering, The University of Texas at San Antonio, San Antonio, USA

c Department of Physics and Astronomy, The University of Texas at San Antonio, San Antonio, USA

d School of Agronomic Sciences - IBAM-CONICET, National University of Cuyo, Mendoza, Argentina

Abstract

This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.

Graphical abstract: Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

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Article information

DOI
https://doi.org/10.1039/C1RA00198A
Article type
Paper
Submitted
23 May 2011
Accepted
22 Jul 2011
First published
31 Aug 2011

RSC Adv., 2011,1, 706-714

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Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

K. Y. Chumbimuni-Torres, R. E. Coronado, A. M. Mfuh, C. Castro-Guerrero, M. F. Silva, G. R. Negrete, R. Bizios and C. D. Garcia, RSC Adv., 2011, 1, 706 DOI: 10.1039/C1RA00198A

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