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Issue 7, 2011
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Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

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Abstract

Facile immobilization of horseradish peroxidase (HRP) and glucose oxidase (GOX) was achieved on electrospun nanofibres made of an alternating copolymer of styrene (Sty) and maleic anhydride (MAh). The immobilized enzymes were used to perform a cascade reaction oxidizing D-glucose by GOX to yield H2O2 and employing HRP to catalyze the reaction between the generated H2O2 and Ampliflu Red to yield Resorufin. The average activity of the immobilized HRP was determined to be 21% of that of the free enzyme.

Graphical abstract: Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

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Article information


Submitted
08 Feb 2011
Accepted
15 Mar 2011
First published
26 Mar 2011

Polym. Chem., 2011,2, 1479-1481
Article type
Communication

Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

W. J. Cloete, C. Adriaanse, P. Swart and B. Klumperman, Polym. Chem., 2011, 2, 1479
DOI: 10.1039/C1PY00069A

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