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Issue 7, 2011
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Block copolymer conjugates prepared by sequentially grafting from proteinsvia RAFT

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Abstract

Well-defined thermoresponsive block copolymer conjugates were prepared by two consecutive grafting-from polymerizationsvia reversible addition–fragmentation chain transfer (RAFT) polymerization. A RAFT agent containing an activated ester was conjugated to amines on lysozyme, and N-isopropylacrylamide was polymerized by grafting directly from the modified protein in aqueous buffer. Retention of the active thiocarbonylthio moieties on the ω end of the conjugated chains allowed chain extension viapolymerization of N,N-dimethylacrylamide to yield lysozyme–poly(N-isopropylacrylamide)-b-poly(N,N-dimethylacrylamide) (LYS–PNIPAM-b-PDMA) block copolymer conjugates. Analysis of the conjugates, as well as the cleaved homopolymers and block copolymers, indicated the grafting-from polymerizations were well controlled and that chain extension during block copolymerization was efficient. The temperature-responsive aqueous solution behavior of the resulting conjugates was investigated.

Graphical abstract: Block copolymer conjugates prepared by sequentially grafting from proteinsvia RAFT

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Publication details

The article was received on 20 Jan 2011, accepted on 09 Feb 2011 and first published on 01 Mar 2011


Article type: Paper
DOI: 10.1039/C1PY00031D
Polym. Chem., 2011,2, 1531-1535

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    Block copolymer conjugates prepared by sequentially grafting from proteinsvia RAFT

    H. Li, M. Li, X. Yu, A. P. Bapat and B. S. Sumerlin, Polym. Chem., 2011, 2, 1531
    DOI: 10.1039/C1PY00031D

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