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Issue 1, 2011
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Rapid chemical ligation of oligonucleotides by the Diels–Alder reaction§

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Abstract

Extremely fast and efficient Diels–Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology.

Graphical abstract: Rapid chemical ligation of oligonucleotides by the Diels–Alder reaction

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Article information


Submitted
19 Jul 2010
Accepted
05 Oct 2010
First published
11 Nov 2010

Org. Biomol. Chem., 2011,9, 232-235
Article type
Paper

Rapid chemical ligation of oligonucleotides by the Diels–Alder reaction

A. H. El-Sagheer, V. V. Cheong and T. Brown, Org. Biomol. Chem., 2011, 9, 232 DOI: 10.1039/C0OB00451K

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