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Issue 6, 2011
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Transcriptional response of Streptococcus pneumoniae to Zn2+ limitation and the repressor/activator function of AdcR

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Abstract

Zinc (Zn2+) is an important trace metal ion that has been shown to regulate the expression of several (virulence) genes in streptococci. Previously, we analyzed the genome-wide response of S. pneumoniae to Zn2+-stress. In this work, we have performed a transcriptomic analysis to identify genes that are differentially expressed under intracellular Zn2+ limitation. This revealed a number of genes that are highly upregulated in the absence of extracellular Zn2+, amongst which the genes belonging to the regulon of the Zn2+-responsive repressor AdcR, like adcBCA, encoding a Zn2+-dependent ABC-uptake system, adcAII, encoding a Zn2+-binding lipoprotein, and also virulence genes belonging to the Pht family (phtA, phtB, phtD and phtE). Using transcriptome analysis, lacZ-reporter studies, in vitro DNA binding experiments, and in silico operator predictions, we show that AdcR directly represses the promoters of adcRCBA, adcAII-phtD, phtA, phtB and phtE in the presence of Zn2+. AdcR can also function as an activator, since in the presence of Zn2+ it directly induces expression of adh that encodes a Zn2+-containing alcohol dehydrogenase. In conclusion, the genome-wide transcriptional response of S. pneumoniae to Zn2+ limitation was established, which is mainly mediated via direct regulation by the Zn2+-dependent regulator AdcR.

Graphical abstract: Transcriptional response of Streptococcus pneumoniae to Zn2+ limitation and the repressor/activator function of AdcR

  • This article is part of the themed collection: Zinc
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Publication details

The article was received on 04 Mar 2011, accepted on 03 May 2011 and first published on 21 May 2011


Article type: Paper
DOI: 10.1039/C1MT00030F
Metallomics, 2011,3, 609-618

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    Transcriptional response of Streptococcus pneumoniae to Zn2+ limitation and the repressor/activator function of AdcR

    S. Shafeeq, T. G. Kloosterman and O. P. Kuipers, Metallomics, 2011, 3, 609
    DOI: 10.1039/C1MT00030F

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