Issue 7, 2011
From the journal:

Molecular BioSystems

Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis†

Daniel C. Prophetera  and  Lara K. Mahal*ab  

* Corresponding authors

a Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, USA

b Department of Chemistry, New York University, 100 Washington Sq. E., Room 1001, NY, USA
E-mail: lkmahal@nyu.edu

Abstract

Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.

Graphical abstract: Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

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Article information

DOI
https://doi.org/10.1039/C1MB05047H
Article type
Communication
Submitted
03 Feb 2011
Accepted
04 May 2011
First published
20 May 2011

Mol. BioSyst., 2011,7, 2114-2117

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Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis

D. C. Propheter and L. K. Mahal, Mol. BioSyst., 2011, 7, 2114 DOI: 10.1039/C1MB05047H

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