Jump to main content
Jump to site search

Issue 19, 2011
Previous Article Next Article

Polyelectrolyte multilayers generated in a microfluidic device with pH gradients direct adhesion and movement of cells

Author affiliations

Abstract

In this study, multilayers from polyethylene imine, heparin and chitosan are prepared at three different pH values of 5, 7 and 9. Water contact angle and quartz microbalance measurements show that resulting multilayers differ in terms of wetting behaviour, layer mass and mechanical properties. The multilayer is then formed within a gradient generation microfluidic (μFL) device. Polyethylene imine or heparin solutions of pH 5 are introduced into one inlet and the same solutions but at pH 9 into another inlet of the μFL device. The pH gradient established during the multilayer formation can be visualized inside the microchamber by pH sensitive fluorophores and confocal laser scanning microscopy. From this setup it is expected that properties of multilayers displayed at distinct pH values can be realised in a gradient manner inside the μFL device. Behaviour of the osteoblast cell line MG-63 seeded and cultured on top of multilayers created inside the μFL device support this hypothesis. It is observed that more cells adhere and spread on multilayers build-up at the basic side of the μFL channel, while those cells on top of multilayers built at pH 5 are fewer and smaller. These results are consistent with the behaviour of MG-63 cells seeded on multilayers formed at discrete pH values. It is particularly interesting to see that cells start to migrate from multilayers built at pH 5 to those built at pH 9 during 6 h of culture. Overall, the presented multilayer formation setup applying pH gradients leads to surfaces that promote migration of cells.

Graphical abstract: Polyelectrolyte multilayers generated in a microfluidic device with pH gradients direct adhesion and movement of cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 12 May 2011, accepted on 18 Jul 2011 and first published on 18 Aug 2011


Article type: Paper
DOI: 10.1039/C1LC20408D
Lab Chip, 2011,11, 3326-3335

  •   Request permissions

    Polyelectrolyte multilayers generated in a microfluidic device with pH gradients direct adhesion and movement of cells

    K. Kirchhof, A. Andar, H. B. Yin, N. Gadegaard, M. O. Riehle and T. Groth, Lab Chip, 2011, 11, 3326
    DOI: 10.1039/C1LC20408D

Search articles by author

Spotlight

Advertisements