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Issue 19, 2011
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Coalescence-assisted generation of single nanoliter droplets with predefined composition

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Abstract

We demonstrate the generation of highly accurate nanoliter droplets with a predefined composition. This composition control over a single droplet is achieved by merging two droplets with known concentrations and defined volumes. A forced coalescence is accomplished by synchronizing two piezoelectric-based active droplet generators. A microscope-mounted CCD camera is used to record, quantify and monitor the process to assure its high fidelity. The device is disposable, surfactant free, simple to operate and does not require microelectrode fabrication. It delivers a single on-demand droplet with adjustable high resolution mixing ratios up to 9 at a volume range of 1–10 nanoliters. The presented platform offers, for the first time, a means to perform droplet-based high-throughput screening in the nanoliter range.

Graphical abstract: Coalescence-assisted generation of single nanoliter droplets with predefined composition

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Publication details

The article was received on 28 Dec 2010, accepted on 13 Jul 2011 and first published on 09 Aug 2011


Article type: Paper
DOI: 10.1039/C0LC00730G
Lab Chip, 2011,11, 3225-3230

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    Coalescence-assisted generation of single nanoliter droplets with predefined composition

    J. Shemesh, A. Nir, A. Bransky and S. Levenberg, Lab Chip, 2011, 11, 3225
    DOI: 10.1039/C0LC00730G

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