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Issue 6, 2011
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A microfabricated deformability-based flow cytometer with application to malaria

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Abstract

Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated “deformability cytometer” that measures dynamic mechanical responses of 103 to 104 individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers, and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner.

Graphical abstract: A microfabricated deformability-based flow cytometer with application to malaria

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Article information


Submitted
03 Oct 2010
Accepted
16 Dec 2010
First published
03 Feb 2011

Lab Chip, 2011,11, 1065-1073
Article type
Paper

A microfabricated deformability-based flow cytometer with application to malaria

H. Bow, I. V. Pivkin, M. Diez-Silva, S. J. Goldfless, M. Dao, J. C. Niles, S. Suresh and J. Han, Lab Chip, 2011, 11, 1065
DOI: 10.1039/C0LC00472C

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