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Issue 21, 2011
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Isolation of DNA aptamers using micro free flow electrophoresis

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Abstract

A micro free flow electrophoresis (μFFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of μFFE allowed 1.8 × 1014 sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of μFFE selection.

Graphical abstract: Isolation of DNA aptamers using micro free flow electrophoresis

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Publication details

The article was received on 27 May 2011, accepted on 05 Sep 2011 and first published on 23 Sep 2011


Article type: Paper
DOI: 10.1039/C1LC20461K
Lab Chip, 2011,11, 3703-3709

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    Isolation of DNA aptamers using micro free flow electrophoresis

    M. Jing and M. T. Bowser, Lab Chip, 2011, 11, 3703
    DOI: 10.1039/C1LC20461K

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