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Issue 14, 2011
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The construction of an individually addressable cell array for selective patterning and electroporation

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Abstract

In basic cell biology research and drug discovery, it is important to rapidly introduce genes, proteins or drug compounds into cells without permanent damage. Here, we report a three dimensional SU-8 micro-well structure sandwiched with an indium tin oxide (ITO) electrode-covered slide from the top and an individually addressable array of microelectrodes on the bottom to allow parallel delivery of exogenous molecules into various cells in a spatially specific manner. A positive dielectrophoretic force was selectively applied by energizing appropriate electrodes to capture the dispersed cells at the bottom electrode, while the micro-wells were designed to confine cellsin situ when the positive dielectrophoretic force is removed. The combination of spatial positive dielectrophoresis (pDEP) and micro-wells made it possible to construct cell microarrays with specific patterns. Once the cells become attached to the electrodes, different plasmids can be introduced sequentially for selective electroporation. The present cell arraying-assisted electroporation chip integrates a pDEP-assisted cell positioning function with selective electroporation to provide a simple and efficient method for gene transfer. This platform is ideal for high throughput screening of compounds in parallel and thus holds promise for applications in cellular and molecular research.

Graphical abstract: The construction of an individually addressable cell array for selective patterning and electroporation

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Publication details

The article was received on 02 Mar 2011, accepted on 26 Apr 2011 and first published on 31 May 2011


Article type: Paper
DOI: 10.1039/C1LC20183B
Lab Chip, 2011,11, 2417-2423

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    The construction of an individually addressable cell array for selective patterning and electroporation

    Y. Xu, H. Yao, L. Wang, W. Xing and J. Cheng, Lab Chip, 2011, 11, 2417
    DOI: 10.1039/C1LC20183B

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