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Issue 14, 2011
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A lab-on-a-chip for rapid blood separation and quantification of hematocrit and serum analytes

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Abstract

In this work, a new lab-on-a-chip for rapid analysis of low volume blood samples was designed, fabricated and demonstrated for integration of serum separation, hematocrit evaluation, and protein quantitation. Blood separation was achieved using microchannel flow-based separation. A novel method for evaluating hematocrit from microfluidic flow-separated blood samples was developed using gray scale analysis of a point-and-shoot digital photograph of separated blood in a micochannel. Protein quantitation was subsequently performed in a high surface area-to-volume ratio microfluidic chemiluminescent immunoassay using cell depleted serum produced by microfluidic flow-based separation of whole blood samples. All three steps were achieved in a single microchannel with separation of blood samples and hematocrit evaluation in less than 1 min, and protein quantitation in 5 min.

Graphical abstract: A lab-on-a-chip for rapid blood separation and quantification of hematocrit and serum analytes

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Publication details

The article was received on 20 Feb 2011, accepted on 16 May 2011 and first published on 08 Jun 2011


Article type: Paper
DOI: 10.1039/C1LC20144A
Lab Chip, 2011,11, 2440-2446

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    A lab-on-a-chip for rapid blood separation and quantification of hematocrit and serum analytes

    A. W. Browne, L. Ramasamy, T. P. Cripe and C. H. Ahn, Lab Chip, 2011, 11, 2440
    DOI: 10.1039/C1LC20144A

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