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Issue 3, 2011
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Hydrophoretic high-throughput selection of platelets in physiological shear-stress range

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Abstract

A gentle, but fast means for low-stress, high-throughput platelet purification is of significant clinical and biotechnological utility. Current implementations to sort platelets, however, require an external physical field, specialized buffer, or the harsh separation condition of high shear stress that tends to cause platelet stimulation. Here we report the use of hydrophoretic size separation in a wider channel and its parallelization to augment its throughput capability, maintaining physiological shear-stress range. We demonstrate a parallelized device comprising 10 stacks of the wide-channel hydrophoresis device, yielding a throughput of 2.9 million cells sāˆ’1 and a platelet purity of 76.8%. The use of the wide channel for hydrophoresis also facilitates clogging-free separation by sorting blood clots and plaques. The wide-channel hydrophoresis offers the potential for gentle, fast, clogging-free sorting of rare blood cells with extreme throughput capabilities.

Graphical abstract: Hydrophoretic high-throughput selection of platelets in physiological shear-stress range

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Article information


Submitted
26 Jun 2010
Accepted
01 Oct 2010
First published
12 Nov 2010

Lab Chip, 2011,11, 413-418
Article type
Paper

Hydrophoretic high-throughput selection of platelets in physiological shear-stress range

S. Choi, T. Ku, S. Song, C. Choi and J. Park, Lab Chip, 2011, 11, 413
DOI: 10.1039/C0LC00148A

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