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Issue 14, 2011
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Self assembly of acetylcholinesterase on a gold nanoparticlesgraphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker

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Abstract

A nanohybrid of gold nanoparticles (Au NPs) and chemically reduced graphene oxide nanosheets (cr-Gs) was synthesized by in situgrowth of Au NPs on the surface of graphene nanosheets in the presence of poly(diallyldimethylammonium chloride) (PDDA), which not only improved the dispersion of Au NPs but also stabilized cholinesterase with high activity and loading efficiency. The obtained nanohybrid was characterized by TEM, XRD, XPS, and electrochemistry. Then an enzyme nanoassembly (AChE/Au NPs/cr-Gs) was prepared by self-assembling acetylcholinesterase (AChE) on Au NP/cr-Gs nanohybrid. An electrochemical sensor based on AChE/Au NPs/cr-Gs was further developed for ultrasensitive detection of organophosphate pesticide. The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors.

Graphical abstract: Self assembly of acetylcholinesterase on a gold nanoparticles–graphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker

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Publication details

The article was received on 12 Oct 2010, accepted on 02 Feb 2011 and first published on 25 Feb 2011


Article type: Paper
DOI: 10.1039/C0JM03441J
J. Mater. Chem., 2011,21, 5319-5325

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    Self assembly of acetylcholinesterase on a gold nanoparticlesgraphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker

    Y. Wang, S. Zhang, D. Du, Y. Shao, Z. Li, J. Wang, M. H. Engelhard, J. Li and Y. Lin, J. Mater. Chem., 2011, 21, 5319
    DOI: 10.1039/C0JM03441J

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