Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 9, 2011
Previous Article Next Article

Sugar functionalised PEGA surfaces support metabolically active hepatocytes

Author affiliations

Abstract

We demonstrate sugar functionalised hydrogel surface coatings which enable the retention of hepatocyte-specific cell function during in vitro culture. The materials were designed to exploit a unique characteristic of hepatocyte biology, with β-galactose moieties displayed to allow cellular adhesion via the specific asialoglycoprotein receptors (ASGP-R) expressed on hepatocyte cell surfaces. Polyethylene glycol acrylamide hydrogel (PEGA) was modified with a galactose containing ligand, lactobionic acid (LA) with D-glucuronic acid (GlcA) used as a non-ASGP-R binding control. FT-IR analysis of the resultant gels revealed that the sugars had been covalently incorporated into the material. Fluorescence labelling was used to quantify the degree of saccharide integration. Cell culture experiments showed that hepatocytes attached preferentially to sugar containing gel coatings, when compared to non-functionalised PEGA controls. It was observed that cells on the LA and GlcA containing surfaces were more metabolically active, compared to controls, and proliferated to a monolayer by day 7 in culture and maintained some characteristics of hepatocyte functionality, such as urea synthesis over the course of 7 days. Despite these observations, further investigations using flow cytometry and RT-PCR, proved that cell attachment was unlikely to involve an ASGP-R mediated mechanism.

Graphical abstract: Sugar functionalised PEGA surfaces support metabolically active hepatocytes

Back to tab navigation

Supplementary files

Article information


Submitted
30 Aug 2010
Accepted
06 Dec 2010
First published
12 Jan 2011

J. Mater. Chem., 2011,21, 2901-2908
Article type
Paper

Sugar functionalised PEGA surfaces support metabolically active hepatocytes

R. F. Ambury, C. L. R. Merry and R. V. Ulijn, J. Mater. Chem., 2011, 21, 2901
DOI: 10.1039/C0JM02874F

Search articles by author

Spotlight

Advertisements