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Issue 5, 2011
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GlutathioneS-transferase as a general and reversible tag for surface immobilization of proteins

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Abstract

The ability to immobilize biomolecules on nanopatterned substrates is important for diverse applications including tissue engineering and proteomics. The design of general linkers can allow a single substrate to be used for multiple purposes, as well as reconfiguration of devices if the linkage is reversible. In this work, the glutathione S-transferase (GST) tag was used to reversibly immobilize green fluorescent protein (GFP) and basic fibroblast growth factor (FGF2) on glutathione (GSH)-functionalized nanopatterns fabricated by electron-beam lithography.

Graphical abstract: Glutathione S-transferase as a general and reversible tag for surface immobilization of proteins

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Supplementary files

Article information


Submitted
23 Jul 2010
Accepted
23 Sep 2010
First published
27 Oct 2010

J. Mater. Chem., 2011,21, 1457-1461
Article type
Paper

Glutathione S-transferase as a general and reversible tag for surface immobilization of proteins

C. M. Kolodziej, C. Chang and H. D. Maynard, J. Mater. Chem., 2011, 21, 1457
DOI: 10.1039/C0JM02370A

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