Jump to main content
Jump to site search

Issue 6, 2011
Previous Article Next Article

Magnetic quantitative immunoanalysis of carcinoembryonic antigen by ICP-MS with mercury labels

Author affiliations

Abstract

The need of analytical methods for absolute quantitative protein analysis spurred research on new developments in recent years. In this paper, a sensitive and selective method based on mercury labeled magnetic immunoassay and inductively coupled plasma mass spectrometry (ICP-MS) detection was proposed for the quantification of carcinoembryonic antigen (CEA) in real human serum samples. Size exclusion chromatography (SEC) combined with ICP-MS was applied for the separation and detection of secondary antibody labeled with mercury and excess mercury. After a complete sandwich-type immunoreaction among primary antibody, CEA and secondary antibody labeled with mercury on magnetic nanoparticles (MNPs), the concentration of CEA was determined by microconcentric nebulizer (MCN)-ICP-MS analysis of mercury ion released from secondary antibody with an acid-dissolution (pH 1.0) step. With consumption of only 50 μL sample solution, the established method presented a limit of detection of 0.041 ng mL−1 for CEA, with the relative standard deviation (RSD) of 5.2% (c = 8 ng mL−1, n = 7). The response for CEA was linear over a dynamic range from 0.1 to 50 ng mL−1. The analytical results of CEA in three different human serum samples obtained by the proposed method are in good agreement with that obtained by the commonly used clinical method of chemiluminescent immunoassay (CLIA). The developed method was versatile and could be easily extended to other proteins quantitative analysis, especially for specific biomarker determination.

Graphical abstract: Magnetic quantitative immunoanalysis of carcinoembryonic antigen by ICP-MS with mercury labels

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Jan 2011, accepted on 04 May 2011 and first published on 12 May 2011


Article type: Paper
DOI: 10.1039/C1JA00007A
J. Anal. At. Spectrom., 2011,26, 1217-1223

  •   Request permissions

    Magnetic quantitative immunoanalysis of carcinoembryonic antigen by ICP-MS with mercury labels

    H. Peng, B. Chen, M. He, Y. Zhang and B. Hu, J. Anal. At. Spectrom., 2011, 26, 1217
    DOI: 10.1039/C1JA00007A

Search articles by author

Spotlight

Advertisements