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The communication describes the synthesis and studies of 9-piperazine substituted perylene-3,4-dicarboximide (1). In aqueous solution, the ratio of fluorescence emission intensities from the localized excited state (∼585 nm) to the charge transfer excited state (∼695 nm) increases in the pH range of 7.5–9. Furthermore, compound 1 may be used for ratiometric detection of double-stranded DNA.

Graphical abstract: 9-Piperazine substituted perylene-3,4-dicarboximide as a fluorescent probe in ratiometric analysis

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