Rapid determination of polyphenols in cut tobacco by microwave-assisted extraction-ultrahigh performance liquid chromatography

Abstract

A fast microwave-assisted extraction-ultrahigh performance liquid chromatography (MAE-UPLC) method was developed for the simultaneous determination of chlorogenic acid, scopoletin and rutin in cut tobacco. The extraction conditions of polyphenols were determined by single-factor and orthogonal experiment. The optimal conditions were found to be as follows: methanol concentration of 60% (v/v), extraction solvent temperature of 90 °C, microwave extraction time of 1 min and solid to liquid ratio of 1 : 20 (g mL⁻¹), the recovery rates of polyphenols in cut tobacco were between 96.5% and 105.6%, relative standard deviations were lower than 5%. The total analysis time for a sample was less than 20 min, the results were satisfactory.