Jump to main content
Jump to site search

Issue 10, 2011
Previous Article Next Article

Rapid determination of polyphenols in cut tobacco by microwave-assisted extraction-ultrahigh performance liquid chromatography

Author affiliations

Abstract

A fast microwave-assisted extraction-ultrahigh performance liquid chromatography (MAE-UPLC) method was developed for the simultaneous determination of chlorogenic acid, scopoletin and rutin in cut tobacco. The extraction conditions of polyphenols were determined by single-factor and orthogonal experiment. The optimal conditions were found to be as follows: methanol concentration of 60% (v/v), extraction solvent temperature of 90 °C, microwave extraction time of 1 min and solid to liquid ratio of 1 : 20 (g mL−1), the recovery rates of polyphenols in cut tobacco were between 96.5% and 105.6%, relative standard deviations were lower than 5%. The total analysis time for a sample was less than 20 min, the results were satisfactory.

Graphical abstract: Rapid determination of polyphenols in cut tobacco by microwave-assisted extraction-ultrahigh performance liquid chromatography

Back to tab navigation

Article information


Submitted
28 Apr 2011
Accepted
30 Jul 2011
First published
15 Sep 2011

Anal. Methods, 2011,3, 2421-2424
Article type
Paper

Rapid determination of polyphenols in cut tobacco by microwave-assisted extraction-ultrahigh performance liquid chromatography

Z. Rui-feng, L. Feng and H. Jing, Anal. Methods, 2011, 3, 2421
DOI: 10.1039/C1AY05248A

Social activity

Search articles by author

Spotlight

Advertisements