In situ analysis of [8-13C-7-15N]-double-labelled theophylline by a triple resonance NMR technique

Koya Yamaguchi; Ryosuke Ueki; Hisatsugu Yamada; Yasuhiro Aoyama; Hiroshi Nonaka; Shinsuke Sando

doi:10.1039/C1AY05100H

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In situ analysis of [8-¹³C-7-¹⁵N]-double-labelled theophylline by a triple resonance NMR technique†

Koya Yamaguchi,^a Ryosuke Ueki,^a Hisatsugu Yamada,^b Yasuhiro Aoyama,^c Hiroshi Nonaka^a and Shinsuke Sando*^ad

Author affiliations

* Corresponding authors

^a INAMORI Frontier Research Center, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Japan
E-mail: ssando@ifrc.kyushu-u.ac.jp

^b Advanced Biomedical Engineering Research Unit, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Japan

^c Department of Molecular Chemistry and Biochemistry, Doshisha University, Kyotanabe, Kyoto, Japan

^d PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, Japan

Abstract

[8-¹³C-7-¹⁵N]-labelled theophylline was synthesized and applied for in situ analysis using a ¹H–{¹³C–¹⁵N} triple resonance NMR technique. The triple resonance analysis enabled selective detection of labelled theophylline in a mixture containing excess amino acids or crude mouse liver extract, where conventional ¹H NMR analysis suffers from difficulty because of background signals.

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Article information

DOI: https://doi.org/10.1039/C1AY05100H
Article type: Paper
Submitted: 23 Feb 2011
Accepted: 05 May 2011
First published: 02 Jun 2011

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Anal. Methods, 2011,3, 1664-1666

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In situ analysis of [8-¹³C-7-¹⁵N]-double-labelled theophylline by a triple resonance NMR technique

K. Yamaguchi, R. Ueki, H. Yamada, Y. Aoyama, H. Nonaka and S. Sando, Anal. Methods, 2011, 3, 1664 DOI: 10.1039/C1AY05100H

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Analytical Methods