Jump to main content
Jump to site search

Issue 12, 2011
Previous Article Next Article

Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

Author affiliations

Abstract

Micellar liquid chromatography (MLC) has been developed for the analysis of opium alkaloids. The composition of micellar mobile phase for the separation of morphine, codeine, papaverine and noscapine have been optimized by a chemometric tool taking into account the efficiency and asymmetry of chromatographic peaks, resolution between each pair of peaks and total time of separation. The optimized mobile phase composition (0.10 M sodium dodecylsulfate (SDS), 5% (v/v) 1-butanol; pH 2.5) allows to fully separate alkaloids within 15 min. The proposed MLC method was validated, compared with the RP-HPLC method and successfully applied for the analysis of the pharmaceutical preparation “Omnopon, solution for injection”.

Graphical abstract: Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

Back to tab navigation

Publication details

The article was received on 29 Jun 2011, accepted on 05 Oct 2011 and first published on 03 Nov 2011


Article type: Paper
DOI: 10.1039/C1AY05389B
Anal. Methods, 2011,3, 2749-2757

  •   Request permissions

    Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

    A. U. Kulikov, A. P. Boichenko and A. G. Verushkin, Anal. Methods, 2011, 3, 2749
    DOI: 10.1039/C1AY05389B

Search articles by author

Spotlight

Advertisements