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Issue 9, 2011
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Assay design considerations for use of affinity aptamer amplification in ultra-sensitive protein assays using capillary electrophoresis

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Abstract

Affinity probe capillary electrophoresis (APCE) holds promise to be a powerful tool in diagnostic applications that rely on the selective and sensitive detection of proteins. Aptamers can serve to unlock the full potential of the technology since their use opens up possibilities to unite the immuno-sensing and separating strength of capillary electrophoresis with the signal enhancement power of nucleic acid amplification. To enable routine use of aptamer APCE, careful selection of the experimental conditions is crucial. Here it is shown how customization and careful consideration of the experimental parameters can significantly improve assay performance.

Graphical abstract: Assay design considerations for use of affinity aptamer amplification in ultra-sensitive protein assays using capillary electrophoresis

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Publication details

The article was received on 06 Mar 2011, accepted on 09 Jun 2011 and first published on 18 Jul 2011


Article type: Technical Note
DOI: 10.1039/C1AY05124E
Anal. Methods, 2011,3, 2156-2159

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    Assay design considerations for use of affinity aptamer amplification in ultra-sensitive protein assays using capillary electrophoresis

    K. P. F. Janssen, K. Knez, J. Pollet, S. J. Roberts, J. Schrooten and J. Lammertyn, Anal. Methods, 2011, 3, 2156
    DOI: 10.1039/C1AY05124E

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