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Issue 18, 2011
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Characterization and application to the detection of single-stranded DNA binding protein of fluorescent DNA-templated copper/silver nanoclusters

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Abstract

A simple strategy for the preparation of strongly fluorescent and stable DNA–Cu/Ag NCs from reduction of AgNO3 and Cu(NO3)2 by NaBH4 in the presence of DNA having a sequence 5′-CCCTTAATCCCC-3′ has been demonstrated. Fluorescence, absorption, X-ray photoelectron spectroscopy (XPS), and electrospray ionization-mass spectrometry (ESI-MS) measurements have been applied to the characterization of the DNA–Cu/Ag NCs. The ESI-MS data reveal that each DNA–Cu/Ag NC contained 2 Ag and 1 Cu atoms. The interactions among DNA with the Ag and Cu atoms are further supported by the data of low-temperature fluorescence. In the presence of Cu2+ ions, the reaction time is 1.5 h, which is much shorter than that (120 h) for the preparation of Ag–DNA NCs that are prepared in a mixture of AgNO3, NaBH4 and DNA without containing Cu2+ ions. Relative to the DNA–Ag NCs, the DNA–Cu/Ag NCs have greater fluorescence (quantum yield 51.2% vs. 11.5%). The DNA–Cu/Ag NCs are highly sensitive and selective for the detection of single-stranded DNA binding protein (SSB), with a linear range 1–50 nM and a limit of detection 0.2 nM at a signal-to-ratio of 3.

Graphical abstract: Characterization and application to the detection of single-stranded DNA binding protein of fluorescent DNA-templated copper/silver nanoclusters

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Supplementary files

Article information


Submitted
31 Mar 2011
Accepted
09 Jun 2011
First published
21 Jul 2011

Analyst, 2011,136, 3623-3628
Article type
Paper

Characterization and application to the detection of single-stranded DNA binding protein of fluorescent DNA-templated copper/silver nanoclusters

G. Lan, W. Chen and H. Chang, Analyst, 2011, 136, 3623 DOI: 10.1039/C1AN15258K

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