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Issue 11, 2011
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Binding assay for cholera toxin based on sequestration electrochemistry using lactose labeled with an electroactive compound

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Abstract

A simple electrochemical binding assay for cholera toxin (CT) was developed using lactose labeled with daunomycin as an electroactive compound. The labeled lactose (LL) was determined with high sensitivity by adsorptive stripping voltammetry (AdSV). The electrochemical behaviors of LL at glassy carbon (GC), plastic formed carbon (PFC) and carbon nanotubes paste (CNTP) electrode were investigated. The CNTP electrode showed the greatest accumulation capacity for LL. The assay for CT based on the sequestration electrochemistry was demonstrated. The binding event of the LL to CT was detected by the decrease in the electrochemical response of daunomycin as an electroactive label without a separation process to remove the free LL from the one bound with CT before any measurements can be made. The detection limit of the CT assay using the CNTP electrode was 0.5 nM (42 ng mL−1).

Graphical abstract: Binding assay for cholera toxin based on sequestration electrochemistry using lactose labeled with an electroactive compound

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Publication details

The article was received on 06 Feb 2011, accepted on 16 Mar 2011 and first published on 14 Apr 2011


Article type: Paper
DOI: 10.1039/C1AN15100B
Analyst, 2011,136, 2373-2378

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    Binding assay for cholera toxin based on sequestration electrochemistry using lactose labeled with an electroactive compound

    H. Kuramitz, S. Miyagaki, E. Ueno, N. Hata, S. Taguchi and K. Sugawara, Analyst, 2011, 136, 2373
    DOI: 10.1039/C1AN15100B

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