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Issue 7, 2011
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Minimum-step immuno-analysis based on continuous recycling of the capture antibody

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Abstract

Most immuno-analytical systems employ antibodies that do not readily dissociate upon binding to its partner antigen (i.e., target analyte; α2-macroglobulin as a model) and, thus, either need to be disposed of after one-time use or be reused after binding has been reset. To achieve a minimum-step analysis, an antibody that is capable of rapidly reversible binding with high affinity to an antigen was investigated in this study. This antibody was immobilized on the surface of a label-free sensor, which was combined with microfluidic channels, to demonstrate its applicability. The antibody was successively reused without a regeneration step under physiological conditions, offered specific analysis in the serum medium, and detected the analyte at concentrations as low as 0.1 ng mL−1, which could further be enhanced by 100-fold. The sensor response reached 95% equilibrium after 8.3 and 14.9 min in average on each dose level for the concentration increase and decrease, respectively. The dynamic range covered a 5 logarithmic analyte concentration. Since the sampling size was in the nanolitre to millilitre range per day under the conditions used and the sensor may retain a long shelf-life, it could potentially be used in a clinical setting for long-term, on-line monitoring of diseases.

Graphical abstract: Minimum-step immuno-analysis based on continuous recycling of the capture antibody

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Article information


Submitted
21 Oct 2010
Accepted
13 Jan 2011
First published
25 Feb 2011

Analyst, 2011,136, 1374-1379
Article type
Paper

Minimum-step immuno-analysis based on continuous recycling of the capture antibody

H. Cho, S. Seo, I. Cho, S. Paek, D. Kim and S. Paek, Analyst, 2011, 136, 1374
DOI: 10.1039/C0AN00811G

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