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Issue 5, 2011
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Solution mixing and the emission of light in flow-cells for chemiluminescence detection

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Abstract

Constructing flow-through reactors for chemiluminescence detection by machining channels into polymer disks has enabled the exploration of new configurations and materials that can improve signal intensity beyond that attainable with the traditional coiled-tubing design. Several approaches to merge reactant solutions were examined: an intersection, chamber or deeper well in the centre of a serpentine configuration flow-cell (directly in front of a photomultiplier tube), or a confluence point outside the detection zone. For several analytically useful, rapid chemiluminescence reactions, the single-inlet flow-cell with external Y-piece was most suitable, but for others (such as KMnO4/Mn(II) with morphine, and [Ir(f-ppy)2BPS] with fluoroquinolones) the dual-inlet configuration provided greater signals. The introduction of central mixing zones with larger widths than the channel reduced the chemiluminescence response. The reversing turns of a serpentine channel promote efficient mixing and greater chemiluminescence intensities than a spiral channel, but increasing the sharpness of the turns created areas of poor solution flow and decreased the chemiluminescence response. Teflon disks impregnated with glass microspheres increased the chemiluminescence signals by 13%–17%, due to the greater reflection of stray light towards the photodetector.

Graphical abstract: Solution mixing and the emission of light in flow-cells for chemiluminescence detection

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Supplementary files

Article information


Submitted
01 Aug 2010
Accepted
15 Nov 2010
First published
02 Dec 2010

Analyst, 2011,136, 913-919
Article type
Paper

Solution mixing and the emission of light in flow-cells for chemiluminescence detection

J. M. Terry, E. M. Zammit, T. Slezak, N. W. Barnett, D. C. Olson, D. K. Wolcott, D. L. Edwards and P. S. Francis, Analyst, 2011, 136, 913
DOI: 10.1039/C0AN00591F

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