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Issue 5, 2011
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A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

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Abstract

Alternative, non-electrochemistry-based technologies for continuous glucose monitoring are needed for eventual use in diabetes mellitus. As part of a programme investigating fluorescent glucose sensors, we have developed fibre-optic biosensors using glucose/galactose binding protein (GBP) labelled with the environmentally sensitive fluorophore, Badan. GBP–Badan was attached via an oligohistidine-tag to the surface of Ni–nitrilotriacetic acid (NTA)-functionalized agarose or polystyrene beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the GBP–Badan-beads. Either GBP–Badan agarose or polystyrene beads were loaded into a porous chamber at the end of a multimode optical fibre. Fluorescence lifetime responses were recorded using pulsed laser excitation, high speed photodiode detection and time-correlated single-photon counting. The maximal response was at 100 mM glucose with an apparent Kd of 13 mM (agarose) and 20 mM (polystyrene), and good working-day stability was demonstrated. We conclude that fluorescence lifetime fibre-optic glucose sensors based on GBP–Badan are suitable for development as clinical glucose monitors.

Graphical abstract: A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

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Article information


Submitted
21 Jun 2010
Accepted
24 Nov 2010
First published
17 Dec 2010

Analyst, 2011,136, 968-972
Article type
Paper

A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

T. Saxl, F. Khan, M. Ferla, D. Birch and J. Pickup, Analyst, 2011, 136, 968
DOI: 10.1039/C0AN00430H

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