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Issue 11, 2010
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Change of the mechanical properties of chondrocytes during expansion culture

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Abstract

Chondrocytes change their morphology and dedifferentiate to fibroblast-like cell during in vitro expansion culture. The phenotype variation may have some effect on the mechanical properties of living chondrocytes. This study was performed to determine the effect of the living chondrocytes’ passage number on their stiffness using atomic force microscopy (AFM). We used freshly isolated (P0) and serially passaged (P1-P5) bovine articular chondrocytes (BACs) and compared the results of Young's modulus measurements with changes in the morphology of the cells. We found that the BACs phenotype variation was accompanied with a decrease in the elastic modulus of the living cells. The change in stiffness was most rapid for the first two passages (P0–P2). The comparison of the mechanical properties of chondrocytes with intact cellular membranes and cells with removed membranes enabled us to conclude that the cellular membrane can play a significant role in the mechanical properties of chondrocytes.

Graphical abstract: Change of the mechanical properties of chondrocytes during expansion culture

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Article information


Submitted
22 Dec 2009
Accepted
15 Mar 2010
First published
05 May 2010

Soft Matter, 2010,6, 2462-2469
Article type
Paper

Change of the mechanical properties of chondrocytes during expansion culture

M. J. Wozniak, N. Kawazoe, T. Tateishi and G. Chen, Soft Matter, 2010, 6, 2462
DOI: 10.1039/B926890A

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