Issue 7, 2010

Fluorescence quenched quinone methide based activity probes – a cautionary tale

Abstract

A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling.

Graphical abstract: Fluorescence quenched quinone methide based activity probes – a cautionary tale

Article information

Article type
Paper
Submitted
01 Oct 2009
Accepted
09 Jan 2010
First published
10 Feb 2010

Org. Biomol. Chem., 2010,8, 1610-1618

Fluorescence quenched quinone methide based activity probes – a cautionary tale

J. D. Sellars, M. Landrum, A. Congreve, D. P. Dixon, J. A. Mosely, A. Beeby, R. Edwards and P. G. Steel, Org. Biomol. Chem., 2010, 8, 1610 DOI: 10.1039/B920443A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements