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Issue 9, 2010
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A genetically encoded photocaged Nε-methyl-l-lysine

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Abstract

A photocaged Nε-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover Nε-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

Graphical abstract: A genetically encoded photocaged Nε-methyl-l-lysine

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Supplementary files

Article information


Submitted
01 Feb 2010
Accepted
12 Mar 2010
First published
30 Mar 2010

Mol. BioSyst., 2010,6, 1557-1560
Article type
Communication

A genetically encoded photocaged Nε-methyl-L-lysine

Y. Wang, B. Wu, Z. Wang, Y. Huang, W. Wan, W. K. Russell, P. Pai, Y. N. Moe, D. H. Russell and W. R. Liu, Mol. BioSyst., 2010, 6, 1557
DOI: 10.1039/C002155E

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