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Issue 4, 2010
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A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

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Abstract

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl-L-lysines into one GFPUV protein in E. coli.

Graphical abstract: A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

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Supplementary files

Article information


Submitted
29 Sep 2009
Accepted
26 Jan 2010
First published
15 Feb 2010

Mol. BioSyst., 2010,6, 683-686
Article type
Communication

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Y. Huang, W. K. Russell, W. Wan, P. Pai, D. H. Russell and W. Liu, Mol. BioSyst., 2010, 6, 683
DOI: 10.1039/B920120C

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