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We show that intact neutral molecules of guanosine 5′-monophosphate can be vaporized by laser desorption when its phosphate group is esterified. The UV and IR spectroscopic measurements of this nucleotide reveal the existence of a novel internal hydrogen-bonding conformation of the phosphate group and guanine moiety.

Graphical abstract: Gas-phase isolation of diethyl guanosine 5′-monophosphate and its conformational assignment

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