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Issue 34, 2010
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Topographic imaging of convoluted surface of live cells by scanning ion conductance microscopy in a standing approach mode

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Abstract

Scanning ion conductance microscopy (SICM) using a nanopipette as a probe and ionic current as a feedback signal was introduced as a novel technique to study live cells in a physiological environment. To avoid contact between the pipette tip and cells during the conventional lateral scanning mode, we adopted a standing approach (STA) mode in which the probe was moved vertically to first approach and then retracted from the cell surface at each measurement point on an XY plane. The STA mode ensured non-contact imaging of the topography of live cells and for a wide range of uneven substrates (500 × 300 μm to 5 × 5 μm). We also used a field-programmable gate array (FPGA) board to enhance feedback distance regulation. FPGA dramatically increased the feedback speed and decreased the imaging time (450 s per image) with enhanced accuracy and quality of live cell images. To evaluate the potential of the STA mode for SICM, we carried out imaging of a convoluted surface of live cell in various scan ranges and estimated the spatial resolutions of these images.

Graphical abstract: Topographic imaging of convoluted surface of live cells by scanning ion conductance microscopy in a standing approach mode

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Publication details

The article was received on 08 Feb 2010, accepted on 22 Apr 2010 and first published on 18 May 2010


Article type: Paper
DOI: 10.1039/C002607G
Citation: Phys. Chem. Chem. Phys., 2010,12, 10012-10017
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    Topographic imaging of convoluted surface of live cells by scanning ion conductance microscopy in a standing approach mode

    Y. Takahashi, Y. Murakami, K. Nagamine, H. Shiku, S. Aoyagi, T. Yasukawa, M. Kanzaki and T. Matsue, Phys. Chem. Chem. Phys., 2010, 12, 10012
    DOI: 10.1039/C002607G

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