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Issue 47, 2010
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Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring

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Abstract

A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with β-1,4-galactosyltransferase.

Graphical abstract: Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring

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Supplementary files

Article information


Submitted
04 Oct 2010
Accepted
21 Sep 2010
First published
25 Oct 2010

Chem. Commun., 2010,46, 8968-8970
Article type
Communication

Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring

M. C. Galan, A. T. Tran and C. Bernard, Chem. Commun., 2010, 46, 8968
DOI: 10.1039/C0CC04224B

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