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Issue 47, 2010
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Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring

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Abstract

A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with β-1,4-galactosyltransferase.

Graphical abstract: Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring

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Publication details

The article was received on 04 Oct 2010, accepted on 21 Sep 2010 and first published on 25 Oct 2010


Article type: Communication
DOI: 10.1039/C0CC04224B
Chem. Commun., 2010,46, 8968-8970

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    Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring

    M. C. Galan, A. T. Tran and C. Bernard, Chem. Commun., 2010, 46, 8968
    DOI: 10.1039/C0CC04224B

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