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Issue 47, 2010
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Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates

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Abstract

Tri-functional activity-based protein probes that encompass an electrophilic trap, a photo-reactive group and a bio-orthogonal ligation handle are described. With these, and in a three-step chemical proteomics approach, proteasomal catalytic sites are covalently and irreversibly modified, followed by photocrosslinking of these to flanking subunits and Staudinger–Bertozzi ligation for visualization and identification of the resulting conjugates.

Graphical abstract: Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates

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Publication details

The article was received on 18 Aug 2010, accepted on 08 Oct 2010 and first published on 27 Oct 2010


Article type: Communication
DOI: 10.1039/C0CC03322G
Chem. Commun., 2010,46, 9052-9054

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    Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates

    P. P. Geurink, B. I. Florea, G. A. Van der Marel, B. M. Kessler and H. S. Overkleeft, Chem. Commun., 2010, 46, 9052
    DOI: 10.1039/C0CC03322G

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