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Issue 47, 2010
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Selective labeling of polypeptides using protein farnesyltransferasevia rapid oxime ligation

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Abstract

An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protein modification provides an alternative to the commonly employed Cu(I)-catalyzed click reaction.

Graphical abstract: Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

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Publication details

The article was received on 17 Aug 2010, accepted on 28 Sep 2010 and first published on 22 Oct 2010


Article type: Communication
DOI: 10.1039/C0CC03305G
Chem. Commun., 2010,46, 8998-9000

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    Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

    M. Rashidian, J. K. Dozier, S. Lenevich and M. D. Distefano, Chem. Commun., 2010, 46, 8998
    DOI: 10.1039/C0CC03305G

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