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Issue 30, 2010
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Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

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Abstract

We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).

Graphical abstract: Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

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Publication details

The article was received on 02 Apr 2010, accepted on 13 May 2010 and first published on 03 Jun 2010


Article type: Communication
DOI: 10.1039/C0CC00735H
Chem. Commun., 2010,46, 5461-5463

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    Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

    W. L. Tang, Z. Li and H. Zhao, Chem. Commun., 2010, 46, 5461
    DOI: 10.1039/C0CC00735H

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