A quantitative analysis of Zidovudine containing formulation by FT-IR and UV spectroscopy

Abstract

In this study IR and UV spectroscopic procedures are describe for the quantitative determination of Zidovudine (AZT) from solid dosage form. For IR spectroscopic method (KBr disc technique) has been used and Ursodeoxycholic acid (UDCA) was used as an internal standard. The specific absorption bands at 2105 and 2931 cm⁻¹ were chosen for AZT and UDCA respectively. In this method, beer's law was obeyed in the concentration range 0.8–2.0% w/w in KBr disc. The regression equation was found to be y = 1.088x + 1.194 with correlation coefficient 0.9819, and the assay was found to be 99.8% with RSD of 2.157%. The UV spectroscopic method was also used in the quantitative determination of AZT in solid dosage form. 266 nm was chosen as λ_max. The regression equation was found to be y = 0.0434x + 0.027, and the correlation coefficient 0.9989. The assay was found to be 99.81% with RSD of 0.273% by the UV spectroscopic method.