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Issue 12, 2010
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Evidence for a stem-cell lineage in corneal squamous cell carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy and multivariate analysis

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Abstract

The cornea is one of the few human tissues where the in situ locations of stem cells (SCs), transient-amplifying (TA) cells and terminally-differentiated (TD) cells have been relatively well localised and characterised. Mid-infrared (IR) (4000–400 cm−1) is absorbed by biological molecules and facilitates the acquisition in the biochemical-cell fingerprint region (1800–900 cm−1) of spectra representative of structure and function. Human cornea derived from normal or squamous cell carcinoma (SCC) samples were acquired, cryosectioned (10 µm), floated onto BaF2 windows and interrogated using synchrotron-based radiation (SRS) Fourier-transform IR (FTIR) microspectroscopy. Spectra were analysed using principal component analysis (PCA) with or without linear discriminant analysis (LDA) to allow cluster analysis of the cell categories. A clear cell lineage emanating from SCs to TA cells to TD cells was noted in normal samples. Within the SCC samples, a small sub-population of the cell-derived spectra pointed to a SC-like phenotype with the vast majority pointing to a TAcell-like character; these cells would tend to be the most proliferative within a tissue. Our findings suggest that SRSFTIR microspectroscopy has the potential to identify and characterise cancer SCs.

Graphical abstract: Evidence for a stem-cell lineage in corneal squamous cell carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy and multivariate analysis

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Supplementary files

Article information


Submitted
14 Jul 2010
Accepted
24 Aug 2010
First published
01 Oct 2010

Analyst, 2010,135, 3120-3125
Article type
Paper

Evidence for a stem-cell lineage in corneal squamous cell carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy and multivariate analysis

J. G. Kelly, T. Nakamura, S. Kinoshita, N. J. Fullwood and F. L. Martin, Analyst, 2010, 135, 3120
DOI: 10.1039/C0AN00507J

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