Issue 7, 2010

Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

Abstract

Amplified potentiometric transduction of DNA hybridization based on using liposome ‘nanocarriers’ loaded with the signaling ions is reported. The liposome-amplified potentiometric bioassay involved the duplex formation, followed by the capture of calcium-loaded liposomes, a surfactant-induced release and highly-sensitive measurements of the calcium signaling ions using a Ca2+ ion-selective electrode (ISE). The high loading yield of nearly one million signaling ions per liposome leads to sub-fmol DNA detection limits. Factors affecting the ion encapsulation efficiency and signal amplification are evaluated and discussed. The influence of the surfactant lysing agent is also examined. Such use of ‘green’ calcium signaling ions addresses the inherent toxicity of Ag and CdS nanoparticle tags used in previous potentiometric bioassays. The new strategy was applied for the detection of low levels of E. coli bacteria. It could be readily extended to trace measurements of other important biomolecules in connection to different biorecognition events. The attractive analytical performance makes liposomes a useful addition to the armory of potentiometric bioassays.

Graphical abstract: Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

Article information

Article type
Paper
Submitted
02 Apr 2010
Accepted
17 May 2010
First published
28 May 2010

Analyst, 2010,135, 1618-1623

Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes

K. Y. Chumbimuni-Torres, J. Wu, C. Clawson, M. Galik, A. Walter, G. Flechsig, E. Bakker, L. Zhang and J. Wang, Analyst, 2010, 135, 1618 DOI: 10.1039/C0AN00198H

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