Issue 2, 2010
From the journal:

Analyst

Detection ofnitric oxide in tissue samples by ESI-MS

Zheng Shen,a   Abigail Webster,a   Kevin J. Welham,a   Charlotte E. Dyer,b   John Greenmanb  and  Stephen J. Haswell*a  

* Corresponding authors

a Department of Chemistry, Cottingham Rd, Hull, UK
E-mail: s.j.haswell@hull.ac.uk
Fax: +44(0)1482466410
Tel: +44(0)1482465469

b Centre for biomedical research, University of Hull, Hull, UK

Abstract

A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chosen as a probe for the detection of NO by mass spectrometry (MS) in biological tissue samples. The o-phenylenediamine group reacts with NO to form a characteristic benzotriazole. The product was identified using electrospray ionization mass spectrometry (ESI-MS) and the method validated within the range of 95–1900 nM. NO levels associated with tissue biopsies (∼10 mg) from rat vasculature and intestine tissue biopsies have been successfully determined. The different rates of NO generated from tissue samples under hypoxic and normoxic conditions have been studied by this simple and sensitive method.

Graphical abstract: Detection of nitric oxide in tissue samples by ESI-MS

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Article information

DOI
https://doi.org/10.1039/B917643H
Article type
Paper
Submitted
27 Aug 2009
Accepted
24 Nov 2009
First published
30 Nov 2009

Analyst, 2010,135, 302-305

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Detection of nitric oxide in tissue samples by ESI-MS

Z. Shen, A. Webster, K. J. Welham, C. E. Dyer, J. Greenman and S. J. Haswell, Analyst, 2010, 135, 302 DOI: 10.1039/B917643H

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