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Issue 16, 2010
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Fluorescent S-layerprotein colloids

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Four different coloured pH dependent biocolloids have been designed with fluorescent S-layer fusion proteins. The intrinsic self assembling capability of the S-layer protein SgsE on silicon dioxide particles lead to crystalline p2 lattice symmetry which was combined with the fluorescent properties of fused cyan ECFP, green EGFP, yellow YFP and red mRFP1. Confocal microscopy was used to monitor the pH dependence of the fluorescent S-layer coating. Electrophoretic measurements were carried out to understand the colloidal behaviour of the S-layer protein coated particles. It was found with flow cytometry that the stable SgsE-ECFP, SgsE-EGFP, SgsE-YFP and SgsE-mRFP bioparticle suspensions lost 50% of their fluorescence emission at the pKa of the chromophores. These novel fluorescent S-layer biocolloids can be used as pH sensors and might have an important role in surface pH determination.

Graphical abstract: Fluorescent S-layer protein colloids

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Article information

19 Feb 2010
06 May 2010
First published
29 Jun 2010

Soft Matter, 2010,6, 3809-3814
Article type

Fluorescent S-layer protein colloids

B. Kainz, K. Steiner, U. B. Sleytr, D. Pum and J. L. Toca-Herrera, Soft Matter, 2010, 6, 3809
DOI: 10.1039/C0SM00008F

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